Nalge Nunc NunclonDELTA MultiDishes 140675 Review

Is the Hype Real? Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 Reviewed

In the world of cell culture and biological research, reliable and consistent tools are not just helpful, they are essential. The Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75, from Nalge Nunc, promises exactly that: a sterile, optimized environment for cell attachment and growth, all in a convenient multi-well format. But with so many options available, does this product truly live up to the demands of a rigorous lab setting?

My journey to these multi-dishes stemmed from a very real need in my consulting work. Specifically, I was tasked with optimizing a high-throughput screening assay for a pharmaceutical client and needed a reliable multi-well plate for culturing cells under consistent conditions. The alternatives were inconsistent well-to-well cell growth which directly affected the results and conclusions.

Upon receiving the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75, my initial impression was dominated by the meticulous packaging. Each dish was individually sleeved within the larger case, a clear indication of the manufacturer’s commitment to sterility. Compared to standard, non-treated polystyrene dishes, these felt sturdier, and the alphanumeric markings were noticeably clearer than those on competitive products from Corning and Greiner Bio-One that I’ve used previously. The keyed lids felt like a welcome addition.

My initial reaction was definitely one of cautious optimism. I was eager to see if these multi-dishes could provide the consistent results I needed and whether the NunclonDELTA surface treatment would truly enhance cell attachment and growth.

Real-World Testing: Putting Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 to the Test

First Use Experience

My initial testing involved culturing mammalian cells (specifically, HEK293 cells) in the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 for a dose-response assay. The dishes were tested in a humidified incubator at 37°C with 5% CO2. The real test came with monitoring cell adhesion and growth over 72 hours.

Even with changes in humidity within the incubator, the design of the lid prevented noticeable condensation. I did not encounter any well-to-well contamination or evidence of non-sterile conditions. The cells adhered rapidly and spread evenly across the NunclonDELTA treated surface.

Extended Use & Reliability

After several weeks of using the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 for various cell-based assays, including cytotoxicity and proliferation studies, the reliability has been truly impressive. There have been no signs of degradation in the polystyrene or any compromise in sterility, which is paramount. The alphanumeric markings also held up well, even after repeated handling and labeling.

Maintenance is straightforward, as the dishes are designed for single-use disposal. Compared to using standard, non-treated dishes, the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 consistently provided better cell attachment, more uniform cell growth, and reduced variability in assay results, which made all the difference.

Breaking Down the Features of Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75

Specifications

The Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 are designed specifically for cell culture applications, and their specifications reflect this:

• Number of Wells: 6 – This is ideal for assays requiring multiple replicates or different treatment conditions.
• Well Area: 9.6 cm² – This provides ample surface area for cell attachment and growth in each well.
• Working Volume: 2.0–5.0 mL – A versatile volume range allows for various assay types and incubation times.
• External Dimensions: 128 x 86 mm – A standard size facilitates compatibility with automated liquid handling systems and microscopes.
• Dishes Per Sleeve: 1 – This individual packaging ensures sterility until the moment of use.
• Material: Polystyrene with NunclonDELTA surface treatment – This enhances cell attachment and growth compared to standard polystyrene.

These specifications directly impact performance. The NunclonDELTA treatment, in particular, creates a more hydrophilic surface, promoting cell adhesion and spreading, which is crucial for reliable and reproducible results.

Performance & Functionality

The Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 excels in providing a consistent and sterile environment for cell culture. Cell adhesion is rapid and uniform, leading to reliable results in downstream assays. One key strength is the minimal edge effect, where cells near the edge of the well behave differently, often observed in inferior products.

However, the cost per dish can be a limiting factor for labs with tight budgets. Overall, these dishes meet and often exceed the expectations for cell culture multi-well plates.

Design & Ergonomics

The design of the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 is well-considered and optimized for laboratory use. The polystyrene construction strikes a good balance between durability and weight. The alphanumeric markings are clear and easy to read, which is essential for accurate sample tracking. The keyed lids are specifically designed to reduce contamination and provide a snug fit.

The dishes require minimal learning curve, being similar to other standard multi-well plates.

Durability & Maintenance

Designed for single-use applications, the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 offer excellent durability for their intended lifespan. As long as they are stored in a clean, dry environment, they will maintain their integrity until used. No maintenance is required, as they are disposable.

Accessories and Customization Options

The Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 are primarily designed for cell culture. While they don’t offer direct customization options, they are highly compatible with a wide range of accessories commonly used in cell culture labs. These include automated liquid handlers, microscopes, plate readers, and various cell culture media and reagents.

They also work well with standard plate sealers if longer incubation times are needed.

Pros and Cons of Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75

Who Should Buy Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75?

The Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 are perfect for researchers in cell biology, molecular biology, and drug discovery who require a reliable and consistent platform for cell culture. They are particularly beneficial for labs running high-throughput screening assays or those working with sensitive cell lines that require optimal adhesion.

Labs with tight budgets or those focused on reducing plastic waste might want to explore alternative options.

A must-have accessory is a reliable cell culture incubator with precise temperature and CO2 control to ensure optimal cell growth.

Conclusion on Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75

In summary, the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 deliver on their promise of providing a superior environment for cell culture. The NunclonDELTA surface treatment, combined with the sterile packaging and user-friendly design, makes these dishes a valuable tool for any cell-based research lab.

While the price point may be a concern for some, the enhanced cell attachment, consistent results, and reduced variability justify the investment for critical applications.

I would highly recommend these multi-dishes to any researcher looking to improve the reliability and reproducibility of their cell culture experiments. If you value consistency and accuracy in your cell-based assays, the Nalge Nunc NunclonDELTA MultiDishes, Sterile 140675, Case of 75 are a worthwhile investment.