Does the Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 Deliver?
The Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 is more than just a lid; it’s a critical component in the world of molecular biology, ensuring the integrity of PCR reactions. Eppendorf, a renowned name in laboratory equipment, promises purity and reliability with this product, crucial for sensitive research and diagnostics. These lids are certified PCR clean, guaranteeing freedom from DNA, DNase, RNase, and PCR inhibitors, safeguarding against contamination that can derail experiments and skew results.
My background in tactical gear might seem unrelated, but precision and reliability are paramount in both fields; a failed experiment due to contamination is as detrimental as a malfunctioning piece of equipment in a critical situation. I was seeking a robust, contamination-free solution for a bio-monitoring project involving environmental DNA analysis in remote field locations where maintaining sterile conditions is a constant challenge. Opening the package revealed neatly arranged stacks of the polystyrene lids, each exhibiting a uniform, pristine appearance, inspiring confidence in their cleanliness.
While I haven’t used similar lids designed for deep-well or microtiter plates in my past endeavors, the Eppendorf reputation preceded them, making them the clear choice over potentially cheaper, less rigorously tested alternatives. The initial impression was one of reassurance, knowing that a reputable manufacturer like Eppendorf stood behind this product.
Real-World Testing: Putting Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 to the Test
First Use Experience
My initial testing of the Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 involved using them to seal PCR plates containing environmental DNA samples collected from various water sources during a backcountry expedition. The lids were used in conjunction with a portable PCR machine powered by solar energy, simulating a real-world field laboratory. Despite varying ambient temperatures and humidity levels, the lids maintained a tight seal, preventing sample evaporation and minimizing the risk of external contamination during the amplification process.
The Eppendorf lids demonstrated excellent performance even under challenging environmental conditions. I observed that the lids were incredibly easy to handle, even with gloved hands, thanks to their rigid construction and ergonomic design. There were no immediate issues, and the lids provided a sense of security that the PCR reactions would be protected from external elements.
Extended Use & Reliability
After several weeks of continuous use in the field, the Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 consistently performed as expected, maintaining a reliable seal and showing no signs of degradation or distortion. The polystyrene material proved to be resistant to cracking or warping, even after repeated cycles of temperature fluctuations and exposure to UV radiation in direct sunlight. The lids have required no specific cleaning or maintenance beyond simply wiping them down with a sterile cloth, highlighting their convenience and durability in demanding environments.
Compared to using parafilm or adhesive seals in previous field projects, the Eppendorf lids offered a significantly more reliable and user-friendly solution, minimizing the risk of accidental contamination and simplifying the workflow. The lids far outperformed my expectations and proved invaluable to my project.
Breaking Down the Features of Eppendorf Plate Lid Pcr Clean Pk/80 0030131517
Specifications
- Catalog Number: 89173-632. This unique identifier ensures accurate ordering and tracking of the product.
- Type: INST/EQUIP 0-499. This classification indicates the product is considered an instrument or piece of equipment within a specific price range.
- Description: PLATE LID, PCR CLEAN, PK/80. This concisely describes the product’s purpose and quantity per package.
- Supplier Number: 0030131517. This is Eppendorf’s internal product code, crucial for ordering and internal tracking.
- Material: Polystyrene. This ensures compatibility with PCR plates and thermal cyclers while offering rigidity and chemical resistance.
- Certification: PCR Clean. This certification guarantees that the lids are free from DNA, DNase, RNase, and PCR inhibitors, minimizing the risk of contamination.
- Quantity: Pack of 80. This provides a sufficient supply for multiple experiments and reduces the frequency of reordering.
These specifications are critical because they directly impact the reliability and accuracy of PCR experiments. The PCR Clean certification is of utmost importance because any contamination can lead to false-positive results and wasted resources.
Performance & Functionality
The Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 excels in its primary function: sealing PCR plates and protecting the contents from contamination. The lids provide a secure and consistent fit, minimizing evaporation and preventing cross-contamination between wells. The product’s strength lies in its simplicity and reliability, providing peace of mind during critical experiments.
A minor potential weakness could be the lack of individual packaging for each lid; while the pack of 80 is convenient, researchers might prefer individually wrapped lids for enhanced sterility in certain ultra-sensitive applications. Overall, the lids meet and exceed expectations for a PCR plate sealing solution.
Design & Ergonomics
The Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 features a simple, yet effective design optimized for ease of use and reliable performance. The polystyrene material provides a sturdy feel, making the lids easy to handle and position on the PCR plates. The lids fit snugly on standard PCR plates, creating a secure seal without requiring excessive force.
The lack of any complex features means there’s virtually no learning curve; the lids are ready to use straight out of the package. The simple design ensures ease of use.
Durability & Maintenance
The polystyrene construction of the Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 provides good durability under normal laboratory conditions. The lids are resistant to cracking and warping, even after repeated handling and exposure to temperature changes. Maintenance is minimal, as the lids are designed for single-use and do not require cleaning.
Proper storage in a clean, dry environment will further extend the lifespan of the lids. The long-term lifespan is expected to be several years.
Accessories and Customization Options
The Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 is primarily designed for use with standard PCR plates and does not offer extensive customization options. The product is inherently compatible with any standard PCR plate designed to accept lids, making it versatile for use with various instruments. There are no accessories included with the product, as it is a simple, self-contained sealing solution.
Pros and Cons of Eppendorf Plate Lid Pcr Clean Pk/80 0030131517
Pros
- PCR Clean Certification: Guaranteed freedom from contaminants, ensuring reliable and accurate PCR results.
- Consistent and Secure Seal: Prevents evaporation and cross-contamination, safeguarding sample integrity.
- Durable Polystyrene Construction: Resistant to cracking and warping, ensuring long-lasting performance.
- Easy to Use: No special handling or preparation required, streamlining workflow.
- Compatible with Standard PCR Plates: Versatile for use with various instruments and applications.
Cons
- Price: $359.99 may be considered expensive compared to non-certified alternatives.
- Non-Reusable: Designed for single-use, generating plastic waste.
Who Should Buy Eppendorf Plate Lid Pcr Clean Pk/80 0030131517?
Perfect for: Molecular biologists, geneticists, diagnostic laboratories, and any researcher or technician who requires reliable and contamination-free PCR reactions. Those working with precious or limited samples will particularly benefit from the guaranteed cleanliness and secure seal of these lids.
Who should skip this product? Laboratories performing non-critical PCR applications where contamination is not a major concern may opt for cheaper alternatives. Those seeking reusable options to minimize plastic waste might explore alternative sealing methods.
Must-have accessories or modifications? None. The Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 is a self-contained solution that requires no additional accessories or modifications.
Conclusion on Eppendorf Plate Lid Pcr Clean Pk/80 0030131517
The Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 is an essential component for any laboratory prioritizing the integrity and reliability of PCR experiments. While the price point may seem high, the guaranteed PCR Clean certification and the peace of mind it provides justify the investment, especially when working with critical samples or sensitive applications. I would personally recommend this product to anyone seeking a dependable and contamination-free solution for sealing PCR plates.
Don’t compromise on the accuracy of your results; invest in the Eppendorf Plate Lid Pcr Clean Pk/80 0030131517 and ensure the success of your PCR experiments.
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