Real Insights into the VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb
The VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb from VWR represents a workhorse in the molecular biology lab, designed for efficient and reliable DNA or protein separation. At a price point of $151.99, it balances affordability with the functionality needed for processing multiple samples in research and diagnostics. The key feature here is its midi size, optimized for medium-throughput electrophoresis, coupled with the included 16-tooth combs enabling you to run many samples at once.
My need arose from optimizing our lab’s workflow to handle an increasing load of DNA fragment analysis. We needed a system that would reduce our turnaround time in genetic screening projects. The VWR Midi 10 seemed like a great balance between throughput and price.
Unboxing was straightforward; the system felt solid, constructed of what appeared to be durable acrylic. The 0.75 mm x 16-Tooth Comb felt robust enough to handle repeated use without warping or breaking.
We were deciding between this and a similar system from Bio-Rad and one from Invitrogen. We chose the VWR because of its cost-effectiveness and favorable reviews regarding its leak-proof design. We also liked the availability of 16-tooth combs right out of the box, saving on additional purchases.
Initial excitement was high; the promise of increased throughput was enticing. The build quality appeared adequate, and the compact footprint was a definite plus in our crowded lab space.
Real-World Testing: Putting VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb to the Test
First Use Experience
The initial testing involved running DNA fragments generated from PCR reactions. We tested the unit with Tris-acetate-EDTA (TAE) buffer under standard voltage conditions. Sample loading was straightforward using the 16-tooth comb.
The runs were consistent, even with different buffer concentrations. We had no issues with buffer leaks, which was a major concern based on our previous experiences with other electrophoresis systems. There was a minor learning curve associated with optimizing voltage and run time to achieve optimal separation, which is pretty typical.
After the first run, we noticed slightly uneven band migration in some lanes, probably due to inconsistent pipetting on our part. However, the overall results were reliable enough for preliminary analysis, and the unit performed as expected.
Extended Use & Reliability
After several weeks of daily use, the VWR Midi 10 system has held up remarkably well. There are no cracks or signs of degradation in the acrylic construction. The platinum electrode cassettes are still intact, showing no sign of corrosion.
Cleaning has been easy; a simple rinse with distilled water after each run has kept the system in good condition. The gel tray and comb are easily cleaned with mild detergent. We’ve noted that occasional staining can occur after extended use but is easily removed with isopropanol.
Compared to our previous system, the VWR Midi 10 has significantly improved our workflow, allowing us to process more samples with greater consistency. We have observed the unit operates with minimal hands-on time once the gel is cast. The gel box holds up well and is a considerable improvement over our prior setup.
Breaking Down the Features of VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb
Specifications
- Description: 0.75 mm x 16-Tooth Comb
- Dimensions: 12.5W x 22L x 9H cm (3 1/2 x 8 1/4 x 3 1/2“)
- Buffer Volume: 300 mL
- Max Sample Capacity: 100
The 16-tooth comb allows for efficient processing of multiple samples, crucial in high-throughput applications. The compact dimensions (12.5W x 22L x 9H cm) of the VWR Midi 10 ensure it occupies minimal bench space.
The 300 mL buffer volume ensures adequate buffer capacity for extended runs without significant ionic depletion. The maximum sample capacity of 100 is especially vital for labs processing numerous samples at once.
Performance & Functionality
The VWR Midi 10 excels in separating DNA or protein fragments effectively, delivering consistent and reliable results. The durable rubber casting gates permit easy, tape-free casting.
The system’s primary strength lies in its ease of use and consistent performance, leading to efficient sample processing. The main weakness stems from the limited gel size, restricting the resolution of closely sized fragments. It generally meets our expectations for a midi-sized electrophoresis unit, providing a robust and reliable separation platform.
Design & Ergonomics
The VWR Midi 10’s build quality is robust, constructed from thick, molded acrylic, ensuring leak-proof performance. It’s lightweight enough for easy relocation, yet sturdy enough to withstand daily use.
The system is user-friendly, requiring minimal setup and offering clear instructions. Novice users can quickly learn to operate the system without significant training, as it features slots in the sides of the trays allow for easy comb placement.
Durability & Maintenance
With proper care and regular cleaning, the VWR Midi 10 should last for years, making it a worthwhile investment. Replacing the platinum electrode cassettes and combs may be necessary over time, but these parts are readily available and reasonably priced.
Maintenance is straightforward; routine cleaning with mild detergents and distilled water prevents buffer buildup and ensures optimal performance. The unit is designed for easy disassembly, further simplifying maintenance and troubleshooting.
Accessories and Customization Options
The system comes with one 10x10cm gel tray, two 10-tooth, 1.5mm thick combs, and well visualization strips. Other comb sizes and configurations are available from VWR to accommodate varying sample numbers and well volumes.
While the system is primarily designed as a standalone unit, users can customize it with external power supplies and gel documentation systems from other brands. Compatibility with standard electrophoresis accessories makes it a versatile platform for various research applications.
Pros and Cons of VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb
Pros
- High Throughput: The 16-tooth combs significantly increase sample processing efficiency.
- Leak-Proof Design: Molded acrylic construction ensures reliable, leak-free operation.
- Easy to Use: Simple setup and operation make it accessible to both novice and experienced users.
- Compact Footprint: Space-saving design ideal for labs with limited bench space.
- Cost-Effective: Excellent performance at an affordable price point.
Cons
- Limited Gel Size: May not be suitable for resolving closely sized fragments.
- Basic Accessories: Only includes basic combs; additional combs must be purchased separately.
Who Should Buy VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb?
The VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb is perfect for molecular biology labs, genetics research facilities, and clinical diagnostic centers requiring medium-throughput DNA or protein separation. The VWR Midi 10 is also great for educational institutions teaching electrophoresis techniques.
This system might not be ideal for labs needing high-resolution separation of very large DNA fragments, where larger gel formats and pulsed-field electrophoresis may be necessary. It also may not be suitable for facilities demanding robotic automation.
A must-have accessory would be a high-quality electrophoresis power supply, as well as a UV transilluminator or gel documentation system. Additional combs of varying tooth sizes would also enhance the system’s versatility.
Conclusion on VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb
In summary, the VWR Midi 10 Electrophoresis System E1110-16-0.7 Combs 0.75 Mm x 16-Tooth Comb is a reliable and cost-effective solution for medium-throughput electrophoresis. The VWR Midi 10 is a solid choice for laboratories prioritizing efficiency and ease of use.
The price of $151.99 is justified by its performance, durability, and the increased throughput enabled by the 16-tooth combs. The VWR Midi 10 improves sample processing efficiency, providing a dependable platform for molecular biology research and diagnostics.
I would definitely recommend the VWR Midi 10 to any lab seeking a reliable, easy-to-use, and cost-effective electrophoresis system. Upgrade your lab’s capabilities and streamline your workflow with the VWR Midi 10.
Read the full article here
